Magnaporthe oryzae infection triggers rice resistance to brown planthopper through the influence of jasmonic acid on the flavonoid biosynthesis pathway.

In agroecosystems, plants are constantly exposed to attack from diverse herbivorous insects and microbes, and infestation with one species may change the plant defense response to other species. In our investigation of the relationships among rice plants, the brown planthopper Nilaparvata lugens (Stål) and the rice blast fungus Magnaporthe oryzae, we observed a significant increase in the resistance of rice treated with rice blast to N. lugens, as evidenced by improved plant survival rates in a small population resistance study. Subsequent transcriptome data analysis revealed that the rice blast fungus can induce the expression of genes in the jasmonic acid (JA) and flavonoid pathways. Similar to the flavonoid pathway, the JA pathway also contains 2 types of genes that exhibit similar and opposite trends in response to N. lugens and rice blast. Among these genes, the osjaz1 mutant and the osmyc2 mutant were phenotypically confirmed to positively and negatively regulate rice resistance to N. lugens and rice blast, respectively. Subsequent mass spectrometry and quantification experiments showed that the exogenous application of methyl jasmonate (MeJA) can induce the accumulation of eriodictyol, naringenin and quercetin, as well as the expression of OsF3H, Os4CL5 and OsCHI in the flavonoid pathway. This suggests a close connection between the JA pathway and the flavonoid pathway. However, OsF3'H, which negatively regulates rice resistance to N. lugens and rice blast, did not show increased expression. Phenotypic and molecular experiments confirmed that OsMYC2 can bind to and inhibit the expression of OsF3'H, thus revealing the mechanism of rice resistance to N. lugens after treatment with rice blast. These findings will deepen our understanding of the interactions among rice, N. lugens and rice blast.

Targeting optimal power consumption: Optimizing operational parameters for orchard furrowing and fertilizing machine.

In response to the problem of excessive power consumption during the furrowing operation of orchard furrowing fertilizer machines, an optimization experiment of furrowing operation parameters for orchard furrowing fertilizer machine was conducted based on discrete element simulations. This research focused on the impact of furrowing device operation parameters on furrowing power consumption under full machine operating conditions. Firstly, a kinematics analysis of the soil granules during cutting was done. The mathematical model of soil granules through three movement processes of rising, detachment, and falling was established to determine the main factors affecting the power consumption of furrowing. Secondly, in assessing the furrowing power consumption, the stability coefficient of the furrowing depth, and the percentage of soil cover, alongside the key parameters of furrowing depth, forward propulsion velocity, and furrowing blade rotation speed, a comprehensive quadratic orthogonal rotation regression experiment was meticulously conducted. It was established that test metrics and test parameters regress. Finally, the test parameters were comprehensively optimized after analyzing each factor's impact on the test metrics. The orchard furrowing fertilizer machine's optimal operating parameters were determined, and the verification test was performed. According to the field test findings, the forward propulsion velocity was 785 m/h, and the furrowing blade rotation speed was 190 r/min when the furrowing depth was 275 mm. At this point, the furrowing power consumption was 2.39 kW, the soil cover percentage was 69.06%, and the furrowing depth stability coefficient was 95.08%. These results were in line with the requirements of orchard furrowing operation. The findings of the study can be utilized as a guide for structural changes to orchard furrowing equipment and the management of furrowing operation parameters.

A hemizygous loss-of-function variant in BCORL1 is associated with male infertility and oligoasthenoteratozoospermia.

Oligoasthenoteratozoospermia (OAT) is a common type of male infertility; however, its genetic causes remain largely unknown. Some of the genetic determinants of OAT are gene defects affecting spermatogenesis. BCORL1 (BCL6 corepressor like 1) is a transcriptional corepressor that exhibits the OAT phenotype in a knockout mouse model. A hemizygous missense variant of BCORL1 (c.2615T > G:p.Val872Gly) was reported in an infertile male patient with non-obstructive azoospermia (NOA). Nevertheless, the correlation between BCORL1 variants and OAT in humans remains unknown. In this study, we used whole-exome sequencing to identify a novel hemizygous nonsense variant of BCORL1 (c.1564G > T:p.Glu522*) in a male patient with OAT from a Han Chinese family. Functional analysis showed that the variant produced a truncated protein with altered cellular localization and a dysfunctional interaction with SKP1 (S-phase kinase-associated protein 1). Further population screening identified four BCORL1 missense variants in subjects with both OAT (1 of 325, 0.31%) and NOA (4 of 355, 1.13%), but no pathogenic BCORL1 variants among 362 fertile subjects. In conclusion, our findings indicate that BCORL1 is a potential candidate gene in the pathogenesis of OAT and NOA, expanded its disease spectrum and suggested that BCORL1 may play a role in spermatogenesis by interacting with SKP1.

Exploring cell aggregation as a defense strategy against perchlorate stress in Chlamydomonas reinhardtii through multi-omics analysis.

Perchlorate (ClO4-) is a type of novel, widely distributed, and persistent inorganic pollutant. However, the impacts of perchlorate on freshwater algae remain unclear. In this study, the response and defense mechanisms of microalgae (Chlamydomonas reinhardtii) under perchlorate stress were investigated by integrating physiological and biochemical monitoring, transcriptomics, and metabolomics. Weighted gene co-expression network analysis (WGCNA) of transcriptome data was used to analyze the relationship between genes and phenotype and screen the key pathways. C. reinhardtii exhibited aggregate behavior when exposed to 100- and 200-mM perchlorate but was restored to its unicellular lifestyle when transferred to fresh medium. WGCNA results found that the "carbohydrate metabolism" and "lipid metabolism" pathways were closely related to cell aggregation phenotype. The differential expression genes (DEGs) and differentially accumulated metabolites (DAMs) of these pathways were upregulated, indicating that the lipid and carbohydrate metabolisms were enhanced in aggregated cells. Additionally, most genes and metabolites related to phytohormone abscisic acid (ABA) biosynthesis and the mitogen-activated protein kinase (MAPK) signaling pathway were significantly upregulated, indicating their crucial roles in the signal transmission of aggregated cells. Meanwhile, in aggregated cells, extracellular polymeric substances (EPS) and lipid contents increased, photosynthesis activity decreased, and the antioxidant system was activated. These characteristics contributed to C. reinhardtii's improved resistance to perchlorate stress. Above results demonstrated that cell aggregation behavior was the principal defense strategy of C. reinhardtii against perchlorate. Overall, this study sheds new light on the impact mechanisms of perchlorate to aquatic microalgae and provides multi-omics insights into the research of multicellular-like aggregation as an adaptation strategy to abiotic stress. These results are beneficial for assessing the risk of perchlorate in aquatic environments.

UV-B radiation increased the sensitivity of Tibetan soil cyanobacterium Loriellopsis cavernicola to the herbicide glyphosate.

The high concentrations of herbicide and UV-B radiation are two stresses for Tibetan soil microorganisms, but there is limited information about the combined effects of herbicide and UV-B radiation on their levels of stress. In this study, the Tibetan soil cyanobacterium Loriellopsis cavernicola was used to investigate the combined inhibitory effect of the herbicide glyphosate and UV-B radiation on the cyanobacterial photosynthetic electron transport through an analysis of the photosynthetic activity, photosynthetic pigments, chlorophyll fluorescence and antioxidant system activity. The results demonstrated that treatment with herbicide or UV-B radiation and the combination of both stresses caused a decrease in the photosynthetic activity, interfered with the photosynthetic electron transport, and caused the accumulation of oxygen radicals and the degradation of photosynthetic pigments. In contrast, the combined treatment of glyphosate and UV-B radiation had a synergistic effect, i.e., the sensitivity of cyanobacteria to glyphosate increased in the presence of UV-B radiation, which caused the photosynthesis of cyanobacteria to have a greater impact. Since cyanobacteria are the primary producers of soil ecosystems, a high intensity of UV-B radiation in the plateau areas could enhance the inhibition of glyphosate on cyanobacteria, which could affect the ecological health and sustainable development of plateau soils.

Dietary palmitoleic acid reprograms gut microbiota and improves biological therapy against colitis.

Magnitude and diversity of gut microbiota and metabolic systems are critical in shaping human health and diseases, but it remains largely unclear how complex metabolites may selectively regulate gut microbiota and determine health and diseases. Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA). Dietary POA repaired gut mucosal barriers, reduced inflammatory cell infiltrations and expressions of TNF-α and IL-6, and improved efficacy of anti-TNF-α therapy in both acute and chronic IBD mouse models. Ex vivo treatment with POA in cultured inflamed colon tissues derived from Crohn's disease (CD) patients reduced pro-inflammatory signaling/cytokines and conferred appreciable tissue repairment. Mechanistically, POA significantly upregulated the transcriptional signatures of cell division and biosynthetic process of Akkermansia muciniphila, selectively increased the growth and abundance of Akkermansia muciniphila in gut microbiota, and further reprogrammed the composition and structures of gut microbiota. Oral transfer of such POA-reprogrammed, but not control, gut microbiota induced better protection against colitis in anti-TNF-α mAb-treated recipient mice, and co-administration of POA with Akkermansia muciniphila showed significant synergistic protections against colitis in mice. Collectively, this work not only reveals the critical importance of POA as a polyfunctional molecular force to shape the magnitude and diversity of gut microbiota and therefore promote the intestinal homeostasis, but also implicates a new potential therapeutic strategy against intestinal or abenteric inflammatory diseases.

Organosilicon Fluorescent Materials.

In the past few decades, organosilicon fluorescent materials have attracted great attention in the field of fluorescent materials not only due to their abundant and flexible structures, but also because of their intriguing fluorescence properties, distinct from silicon-free fluorescent materials. Considering their unique properties, they have found broad application prospects in the fields of chemosensor, bioimaging, light-emitting diodes, etc. However, a comprehensive review focusing on this field, from the perspective of their catalogs and applications, is still absent. In this review, organosilicon fluorescent materials are classified into two main types, organosilicon small molecules and polymers. The former includes fluorescent aryl silanes and siloxanes, and the latter are mainly fluorescent polysiloxanes. Their synthesis and applications are summarized. In particular, the function of silicon atoms in fluorescent materials is introduced. Finally, the development trend of organosilicon fluorescent materials is prospected.

Outer Membrane Vesicles from Acinetobacter baumannii: Biogenesis, Functions, and Vaccine Application.

This review focuses on Acinetobacter baumannii, a Gram-negative bacterium that causes various infections and whose multidrug resistance has become a significant challenge in clinical practices. There are multiple bacterial mechanisms in A. baumannii that participate in bacterial colonization and immune responses. It is believed that outer membrane vesicles (OMVs) budding from the bacteria play a significant role in mediating bacterial survival and the subsequent attack against the host. Most OMVs originate from the bacterial membranes and molecules are enveloped in them. Elements similar to the pathogen endow OMVs with robust virulence, which provides a new direction for exploring the pathogenicity of A. baumannii and its therapeutic pathways. Although extensive research has been carried out on the feasibility of OMV-based vaccines against pathogens, no study has yet summarized the bioactive elements, biological activity, and vaccine applicability of A. baumannii OMVs. This review summarizes the components, biogenesis, and function of OMVs that contribute to their potential as vaccine candidates and the preparation methods and future directions for their development.

MicroRNA-31 mediated by interferon regulatory factor 7 signaling facilitates control of Mycobacterium tuberculosis infection.

Tuberculosis (TB) induced by Mycobacterium tuberculosis (M. tuberculosis) infection remains a global most deadly infectious disease. While development of more effective TB vaccines and therapeutics relies on identifications of true biomarkers designating an immune protection against M. tuberculosis infection, exact protective immune components against M. tuberculosis infection remain largely unidentified. We previously found that severe TB induced remarkable up-regulation of interferon regulatory factor 7 (IRF7) and IRF7-related gene signatures, implicating that some unknown downstream molecules in IRF7 signaling cascades may determine the M. tuberculosis infection outcomes and serve as a protective immune component against M. tuberculosis infection. Indeed, here, we observe that genetic ablation of IRF7 leads to more severe lung pathology, increased M. tuberculosis burdens, impaired differentiation of effector/memory T subsets, and extensively elevated expression of pro-inflammatory cytokines in lungs. Importantly, IRF7 is vital for sustaining expression of PD-1/PD-L1 and PD-1/PD-L1-modulated miRNA-31. Moreover, interventions of miRNA-31 expressions via administration of miRNA-31 agomir reduces lung pathology and bacilli burdens via inducing up-regulation of gene sets involved in biological processes of defense response or cellular and chemical homeostasis in lungs. Thus, this study uncovers previously unrecognized importance and mechanisms of IRF7-mediated miRNA-31 as a protective immune component against M. tuberculosis infection.

Transcriptomic and Physiological Responses of Chlorella pyrenoidosa during Exposure to 17α-Ethinylestradiol.

17α-ethinylestradiol (17α-EE2) is frequently detected in water bodies due to its use being widespread in the treatment of prostate and breast cancer and in the control of alopecia, posing a threat to humans and aquatic organisms. However, studies on its toxicity to Chlorella pyrenoidosa have been limited to date. This study investigated the effects of 17α-EE2 on the growth, photosynthetic activity, and antioxidant system of C. pyrenoidosa and revealed related molecular changes using transcriptomic analysis. The cell density of algae was inhibited in the presence of 17α-EE2, and cell morphology was also altered. Photosynthetics were damaged, while reactive oxygen species (ROS), superoxide dismutase (SOD), and malondialdehyde (MDA) content increased. Further transcriptomic analysis revealed that the pathways of photosynthesis and DNA replication were affected at three concentrations of 17α-EE2, but several specific pathways exhibited various behaviors at different concentrations. Significant changes in differentially expressed genes and their enrichment pathways showed that the low-concentration group was predominantly impaired in photosynthesis, while the higher-concentration groups were biased towards oxidative and DNA damage. This study provides a better understanding of the cellular and molecular variations of microalgae under 17α-EE2 exposure, contributing to the environmental risk assessment of such hazardous pollutants on aquatic organisms.

The gut microbiota mediates protective immunity against tuberculosis via modulation of lncRNA.

The gut-lung axis has been implicated as a potential therapeutic target in lung disorders. While increasing evidence suggests that gut microbiota plays a critical role in regulating host immunity and contributing to tuberculosis (TB) development and progression, the underlying mechanisms whereby gut microbiota may impact TB outcomes are not fully understood. Here, we found that broad-spectrum antibiotics treatment increased susceptibility to Mycobacterium tuberculosis (M. tuberculosis) infection and modulated pulmonary inflammatory responses in mouse M. tuberculosis infection model. We then identified a commensal gut bacteria-regulated lncRNA, termed lncRNA-CGB, which was down-regulated by dysbiosis of gut microbiota during TB infection. Furthermore, we found that Bacteroides fragilis (B. fragilis) was a direct regulator of lncRNA-CGB, and oral administration of B. fragilis enhanced expression of lncRNA-CGB and promoted anti-TB immunity. Genomic knock-out of lncRNA-CGB led to reduced IFN-γ expression and impaired anti-TB immunity, therefore leading to detrimental effects on M. tuberculosis infection. Mechanistically, lncRNA-CGB interacted with EZH2 and negatively regulated H3K27 tri-methylation (H3K27Me3) epigenetic programming, leading to enhanced IFN-γ expression. Thus, this work not only uncovered previously unrecognized importance of gut bacteria-lncRNA-EZH2-H3K27Me3 axis in conferring immune protection against TB but also identified a potential new paradigm to develop a microbiota-based treatment against TB and potentially other diseases.

Ifnar gene variants influence gut microbial production of palmitoleic acid and host immune responses to tuberculosis.

Both host genetics and the gut microbiome have important effects on human health, yet how host genetics regulates gut bacteria and further determines disease susceptibility remains unclear. Here, we find that the gut microbiome pattern of participants with active tuberculosis is characterized by a reduction of core species found across healthy individuals, particularly Akkermansia muciniphila. Oral treatment of A. muciniphila or A. muciniphila-mediated palmitoleic acid strongly inhibits tuberculosis infection through epigenetic inhibition of tumour necrosis factor in mice infected with Mycobacterium tuberculosis. We use three independent cohorts comprising 6,512 individuals and identify that the single-nucleotide polymorphism rs2257167 'G' allele of type I interferon receptor 1 (encoded by IFNAR1 in humans) contributes to stronger type I interferon signalling, impaired colonization and abundance of A. muciniphila, reduced palmitoleic acid production, higher levels of tumour necrosis factor, and more severe tuberculosis disease in humans and transgenic mice. Thus, host genetics are critical in modulating the structure and functions of gut microbiome and gut microbial metabolites, which further determine disease susceptibility.

External rectal prolapse: abdominal or perineal repair for men? A retrospective cohort study.

BACKGROUND: External rectal prolapse is a relatively rare disease, in which male patients account for a minority. The selection of abdominal repair or perineal repair for male patients has rarely been investigated. METHODS: Fifty-one male patients receiving abdominal repair (laparoscopic ventral rectopexy) or perineal repair (Delorme or Altemeier procedures) at the Sixth Affiliated Hospital of Sun Yat-sen University (Guangzhou, China) between March 2013 and September 2019 were retrospectively analysed. We compared the recurrence, complication rate, post-operative defecation disorder, length of stay, and quality of life between the abdominal and perineal groups. RESULTS: Of the 51 patients, 45 had a complete follow-up, with a median of 48.5 months (range, 22.8-101.8 months). A total of 35 patients were under age 40 years. The complication rate associated with abdominal repair was less than that associated with perineal repair (0% vs 20.7%, P = 0.031) and the recurrence rate was also lower (9.5% vs 41.7%, P = 0.018). Multivariate analysis showed that perineal repair (odds ratio, 9.827; 95% confidence interval, 1.296-74.50; P = 0.027) might be a risk factor for recurrence. Moreover, only perineal repair significantly improved post-operative constipation status (preoperative vs post-operative, 72.4% vs 25.0%, P = 0.001). There was no reported mortality in either of the groups. No patient's sexual function was affected by the surgery. CONCLUSIONS: Both surgical approaches were safe in men. Compared with perineal repair, the complication rate and recurrence rate for abdominal repair were lower. However, perineal repair was better able to correct constipation.

Manganese-doped molybdenum oxide boosts catalytic performance of electrocatalytic wet air oxidation at ambient temperature.

Mn-doping strategy was adopted to modify the structure of MoO2 for enhancing its catalytic activity towards room-temperature electrocatalytic wet air oxidation (ECWAO) reaction. A series of Mn-doped MoO2 were prepared on carbon support, and their structures were investigated to elucidate the productive effect of Mn doping on the catalytic activity of MoO2. The incorporation of MnIII/MnII into the MoO2 lattice induced the transformation from MoIV to MoV and created more oxygen vacancies. Such structural modifications promoted the electron transfer of MoO2 through the redox couples between MoVI/MoV/MoIV and MnIII/MnII, and facilitated the transformation from O2 to adsorbed oxygen species on MoO2 surface. As a result, the ECWAO catalytic activities of Mn-doped MoO2/graphite felt (MoO2/GF) outperformed the activity of MoO2/GF. Among the synthesized series, Mn0.066:MoO2/GF exhibited the highest activity with the maximum turnover frequency (TOF) promoted by 59% than the undoped MoO2/GF. Under the catalysis of Mn0.066:MoO2/GF, the ECWAO process obtains mineralization efficiencies generally above 85% in degrading typical pharmaceutics and person care products (PPCPs). These findings are anticipated to open up a new venue in the design and fabrication of highly active catalysts for air oxidation reactions by using the strategy of selective dopant-induced structure modification.

Photosynthesis Responses of Tibetan Freshwater Algae Chlorella vulgaris to Herbicide Glyphosate.

With the development of agriculture and the widespread application of agrichemicals in Tibet, herbicide residues have become a threat to the ecological safety of Tibetan water bodies. Algae, as the producers in the food chain in water bodies, play an important role in aquatic ecosystems. Therefore, the impact of herbicides on Tibetan algae is of great significance for evaluating ecological health and the protection of Tibetan water ecosystems. In this study, we investigated the inhibitory effect of glyphosate, a herbicide, on the photosynthetic system of Chlorella vulgaris, Tibetan algae, by determining chlorophyll fluorescence and the activity of an antioxidant system. The results revealed that glyphosate at low concentration did not affect the photosynthetic activity of C. vulgaris; however, glyphosate at a high concentration significantly inhibited photosynthetic activity and reduced pigment content. Moreover, high levels of glyphosate also decreased photochemical efficiency and electron transport rate and resulted in ROS accumulation, high SOD activity, and lipid peroxidation. These results suggested that glyphosate could decrease the primary production of aquatic ecosystems and influence their performance. Therefore, reducing the herbicide levels could protect the Tibetan aquatic environment and maintain the health of ecosystems.

An exploratory data quality analysis of time series physiologic signals using a large-scale intensive care unit database.

Physiological data, such as heart rate and blood pressure, are critical to clinical decision-making in the intensive care unit (ICU). Vital signs data, which are available from electronic health records, can be used to diagnose and predict important clinical outcomes; While there have been some reports on the data quality of nurse-verified vital sign data, little has been reported on the data quality of higher frequency time-series vital signs acquired in ICUs, that would enable such predictive modeling. In this study, we assessed the data quality issues, defined as the completeness, accuracy, and timeliness, of minute-by-minute time series vital signs data within the MIMIC-III data set, captured from 16009 patient-ICU stays and corresponding to 9410 unique adult patients. We measured data quality of four time-series vital signs data streams in the MIMIC-III data set: heart rate (HR), respiratory rate (RR), blood oxygen saturation (SpO2), and arterial blood pressure (ABP). Approximately, 30% of patient-ICU stays did not have at least 1 min of data during the time-frame of the ICU stay for HR, RR, and SpO2. The percentage of patient-ICU stays that did not have at least 1 min of ABP data was ∼56%. We observed ∼80% coverage of the total duration of the ICU stay for HR, RR, and SpO2. Finally, only 12.5%%, 9.9%, 7.5%, and 4.4% of ICU lengths of stay had ≥ 99% data available for HR, RR, SpO2, and ABP, respectively, that would meet the three data quality requirements we looked into in this study. Our findings on data completeness, accuracy, and timeliness have important implications for data scientists and informatics researchers who use time series vital signs data to develop predictive models of ICU outcomes.

An Imidazole Thione-Modified Polyhedral Oligomeric Silsesquioxane for Selective Detection and Adsorptive Recovery of Au(III) from Aqueous Solutions.

Developing a material toward simultaneous detection and recovery of gold ions (Au(III)) is highly desirable for the economy and the environment. Herein, we report a highly efficient dual-function material for simultaneous Au(III) detection and recovery by simply introducing abundant imidazole thione and thioether groups in one system. This material, that is, an imidazole thione-modified polyhedral oligomeric silsesquioxane (POSS-2), was prepared by a mild reaction of an imidazolium-containing POSS and sulfur at ambient temperature. The POSS-2 suspension in water can rapidly and selectively detect Au(III) with a very low limit of detection of 1.2 ppb by fluorescence quenching or a visualized color change from white to dark orange. POSS-2 can also selectively and efficiently capture Au(III) with a maximum adsorption uptake of 1486.5 mg/g. The adsorption process well fits with the pseudo-second-order kinetic and Langmuir models. The intriguing dual-function performance is better than most of the previous Au(III) probes or adsorbents. The mechanism study reveals that the detection and adsorption behavior are mainly caused by the redox reaction and coordination between imidazole thione and thioether groups and Au(III). Furthermore, POSS-2 was successfully utilized to extract gold without interference from a discard CPU. These results indicate the potential application of the present dual-function material for Au(III) detection and recovery from aqueous solutions. More dual-functional materials could be designed and prepared by this simple strategy.

Porosity-Induced Selective Sensing of Iodide in Aqueous Solution by a Fluorescent Imidazolium-Based Ionic Porous Framework.

Developing a chemosensor for rapid, sensitive, and visual detection of iodide (I-) by a simple synthetic strategy is still challenging. Herein, we report a highly efficient iodide sensor by simply introducing ionic imidazolium groups into the porous network. This sensor, that is, a fluorescent ionic porous framework (IPF), was prepared by the quaternization reaction of octa((benzylchloride)ethenyl)silsesquioxane and 1,4-bis(1H-imidazole-1-yl)benzene and exhibited moderate porosity with a Brunauer-Emmett-Teller surface area of 379 m2 g-1 and blue fluorescence when excited by UV light. The IPF suspension in water can detect I- with high sensitivity and selectivity among various anions and quick response by fluorescence quenching. In contrast to no response toward I- by the linear model compound and the enhanced sensing performance with an increment of porosity, this finding indicates that the porosity of IPF is important for the detection of I- and an inducement of the sensing process. A fluorescent paper sensor was further developed, which shows high efficiency for the visual detection of I- similar to the abovementioned sensor, suggesting its potential in convenient and on-site sensing of I-. In addition, the paper sensor is recyclable with a remarkable fluorescence resuming ratio of 83% after 10 times cycle detection. Moreover, the developed sensor is used for the analysis of real samples. This work represents the first example of the detection of I- by an ionic porous polymer. Compared with conventional iodide sensors, the present sensor does not require unique structures to form the pseudocavity during sensing I- and can easily achieve high efficiency by incorporating ionic hydrogen bond donors into the porous network, indicating the importance of porosity and the feasibility of replacing the pseudocavity with a real cavity (or pore). More iodide sensors with high efficiency can be designed and fabricated by this novel and simple strategy.

Enhanced removal of trichlorfon and Cd(II) from aqueous solution by magnetically separable chitosan beads immobilized Aspergillus sydowii.

Simultaneous removal of heavy metals and organics from wastewater has always been an environmental problem with great concern. In this study, a novel ecofriendly bioborbent, magnetic chitosan beads immobilized Aspergillus sydowii (MCBAs) were synthesized and used to simultaneously remove trichlorfon (TCF) and Cd(II) from aqueous solution. MCBAs showed an increased special surface area (55.38 m2·g-1) through immobilizing A. sydowii and its saturation magnetization reached 14.62 emu·g-1. The equilibrium removal capacities of TCF and Cd(II) were 135.43 mg·g-1 and 56.40 mg·g-1 in the co-system with 200 mg·L-1 TCF and 50 mg·L-1 Cd(II), respectively. The removal capacities of TCF and Cd(II) were strongly depended on the immobilized A. sydowii spore concentration, initial concentrations of TCF and Cd(II), and MCBAs dose. TCF biodegradation intermediates were identified by gas chromatography-mass spectrometry system. Fourier transform infrared spectra displayed that -OH and -NH groups on MCBAs mainly participated in the Cd(II) sequestration and the CO stretching vibration was possibly related to the degradation intermediates of TCF. MCBAs exhibited excellent recyclability upto four cycles. Therefore, MCBAs are suitable and effective for the simultaneous removal of TCF and Cd(II) from wastewater.